We will first run a test plate to determine the best conditions for your samples. Make several dilutions of some or all of your samples (eg. 1 µl, 2 µl, 5 µl or 10 µl) and aliquot them into a half skirt plate (either odd or even rows). We will run that plate and you can determine the dilution that gives you the best signal for your samples. Once the proper amount has been determined we can proceed with your experimental plate.

Plates should be prepared as follows (including test plate):

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